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ACTA1 Antibody

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ACTA1 Antibody

  恒遠生物抗體平臺現已擁有4萬多種抗體(含單抗和多抗),包括人類常用抗體,模式生物類相關抗體、小分子抗體、二抗、標簽抗體及內參抗體等。并以每年4000種的速度在持續增長。

  恒遠生物擁有專業的質控團隊,配備先進實驗儀器,現已成功地建立了ChIP平臺,因此我們的抗體不僅能提供Biotin、FITC、HRP等標記,大部分抗體都能進行ELISA、WB、IHC、IF、IP、ChIP等應用驗證。

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ACTA1 Antibody

 
  • 貨號:
    HYK-200292
  • 規格:
     
  • 圖片:
    • Western Blot
      Positive WB detected in:Hela whole cell lysate,A549 whole cell lysate,Raw264.7 whole cell lysate,NIH/3T3 whole cell lysate,HepG2 whole cell lysate,Rat brain tissue,Rat heart tissue
      All lanes:Actin antibody at 0.95μg/ml
      Secondary
      Goat polyclonal to rabbit IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
    • IHC image of CSB-RA001205A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
    • Immunofluorescence staining of HepG2 cells with CSB-RA001205A0HU at 1:60,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

產品詳情

  • Uniprot No.:
    P68133
  • 基因名:
    ACTA1
  • 別名:
    a actin antibody; ACTA antibody; ACTA1 antibody; ACTA2 antibody; ACTC antibody; ACTC1 antibody; Actin antibody; ACTS_HUMAN antibody; ACTSA antibody; Alpha 2 actin antibody; alpha skeletal muscle antibody; Alpha-actin-1 antibody; Cardiac muscle alpha actin 1 antibody; Skeletal muscle alpha actin 1 antibody
  • 反應種屬:
    Human
  • 免疫原:
    A synthesized peptide
  • 免疫原種屬:
    Homo sapiens (Human)
  • 標記方式:
    Non-conjugated
  • 克隆類型:
    Monoclonal
  • 抗體亞型:
    Rabbit IgG
  • 純化方式:
    Affinity-chromatography
  • 濃度:
    It differs from different batches. Please contact us to confirm it.
  • 保存緩沖液:
    Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • 產品提供形式:
    Liquid
  • 應用范圍:
    ELISA, WB, IHC, IF, FC
  • 推薦稀釋比:
    Application Recommended Dilution
    WB 1:500-1:5000
    IHC 1:50-1:500
    IF 1:30-1:200
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunohistochemistry (IHC) Protocol
    Immunofluorescence (IF) Protocol
    Flow cytometry (FC) Protocol
  • 儲存條件:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 貨期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.


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